Dual Transfection Strategies: Co-Delivery of siRNA and Reporter Plasmids in Pancreatic Lines
Studying gene regulation in pancreatic cancer often necessitates co-delivery of functional siRNAs and reporter plasmids. This dual transfection strategy enables simultaneous knockdown of target genes and monitoring of downstream transcriptional activity.
Altogen’s high-performance transfection reagents are designed for co-transfection in sensitive lines like Capan-1, BxPC-3, and MIA PaCa-2. By carefully optimizing the reagent-to-DNA:RNA ratio, researchers can achieve co-delivery efficiencies exceeding 75%, even in low-proliferating or tightly adherent epithelial cells.
This approach is particularly useful in studies involving signaling pathways such as NF-κB, Wnt/β-catenin, or TGF-β. For instance, silencing SMAD4 and co-transfecting a luciferase-tagged promoter construct can unravel how TGF-β signaling changes during oncogenesis or treatment response.
Using fluorescent reporters or bioluminescent readouts, downstream gene activity can be monitored in real time, allowing kinetic analysis. These experiments accelerate understanding of gene-gene interactions and support high-throughput drug screening workflows in pancreatic cancer models.