Pancreatic cell lines are indispensable in vitro platforms for probing islet biology, exocrine function, and oncogenic pathways in pancreatic ductal adenocarcinoma (PDAC). Among the most frequently employed human pancreatic cancer cell lines are PANC-1, BxPC-3, MIA PaCa-2, AsPC-1, and Capan-1. Each line exhibits unique genetic and phenotypic characteristics: PANC-1 cells (KRAS^G12D mutant) model ductal adenocarcinoma…
Gene therapy is a promising and rapidly evolving field of medical research that aims to treat or prevent diseases by introducing, modifying, or repairing genetic material within a patient’s cells. It offers a potential solution for various genetic disorders, certain types of cancer, and some viral infections. Gene therapy can be broadly categorized into two…
Biopharmaceuticals are a class of therapeutic drugs derived from biological sources, such as living organisms or their components. They include proteins, nucleic acids, and complex carbohydrates, as well as living cells and tissues. Biopharmaceuticals are distinct from traditional small-molecule drugs, which are chemically synthesized. Due to their complex nature and biological origin, biopharmaceuticals often have…
Personalized medicine, also known as precision medicine, is an emerging approach to healthcare that tailors prevention, diagnosis, and treatment strategies to an individual patient’s unique genetic, environmental, and lifestyle factors. Genomics, the study of an organism’s complete set of DNA, plays a crucial role in enabling personalized medicine by providing insights into a person’s genetic…
Hematological toxicity studies are preclinical studies that are conducted to evaluate the potential adverse effects of a substance on the blood and hematopoietic system. These studies are typically conducted in laboratory animals, such as mice, rats, or dogs, to assess the safety of a drug or other substance before testing in humans. The primary objective…
What are Pharmacokinetics and pharmacodynamics? Pharmacokinetics and pharmacodynamics are two terms that are often used interchangeably in the medical field. Pharmacokinetics is the study of how a drug is absorbed, distributed, metabolized, and excreted by the body. Pharmacodynamics is the study of how a drug affects the body. The influence of pharmacokinetics and pharmacodynamics on…
Best practices for GLP compliance in pharmacology and toxicology studies involve adhering to the fundamental principles of Good Laboratory Practice. These practices help ensure the generation of high-quality, reliable, and reproducible data that can be used for regulatory submissions and decision-making. Below are some best practices for GLP compliance in pharmacology and toxicology studies: By…
Pulmonary toxicity studies are preclinical studies that are conducted to evaluate the potential adverse effects of a substance on the respiratory system, including the lungs and airways. These studies are typically conducted in laboratory animals, such as rats, mice, or dogs, to assess the safety of a drug or other substance before testing in humans….
Pancreatic beta-cell-specific gene delivery holds promise for the treatment of type 2 diabetes, a metabolic disorder characterized by insulin resistance and impaired beta-cell function. Targeted gene delivery to pancreatic beta cells aims to enhance their function, increase insulin secretion, improve glucose regulation, and potentially restore normal glucose homeostasis. Here’s an overview of the potential strategies…
Gene editing strategies offer promising avenues for the treatment of monogenic forms of diabetes, which are caused by specific genetic mutations affecting pancreatic function and insulin regulation. Here’s an overview of gene editing strategies that can be employed for the treatment of monogenic forms of diabetes: CRISPR-Cas9-Mediated Gene Editing: CRISPR-Cas9 is a powerful gene editing…
